Although we have posted about this covert project a while ago, but we are still shocked at the recent announcement from the global geopolitical player China that they have proceeded with the experimental genome research that will create the opportunity for the weakening of the natural defense mechanism of a preselected species in favor of another one.
The project has been sanctioned by the Academy of Sciences, National Academy of Medicine, The Royal Society, etc…
CRISPR babies: new details on the experiment that shocked the world
By Michael Le Page
On Monday, the world was stunned by an Associated Press story claiming that the first gene-edited babies had been born in China. On Wednesday, the scientist responsible revealed far more details during a talk at a gene-editing summit in Hong Kong, including that there is another pregnancy.
There hasn’t yet been any independent verification that two gene-edited girls really have been born. But the technical details revealed by He Jiankui today may have been enough to convince many of the scientists in attendance. However, questions still remain over the ethics and safety of the experiment.
The stated aim of the project was to make individuals immune to HIV by disabling the gene for a protein called CCR5, which is exploited by the virus. However, disabling this gene does not provide complete protection against HIV and the broader consequences of knocking out this gene – which is involved in immune function – are unclear.
The team began by using the CRISPR gene editing method to disable CCR5 in mice and monkeys, He said, and found no health or behavioural issues. But one of the organisers of the summit, Robin Lovell-Badge of the Francis Crick Institute in London, pointed out that immune genes affect the entire body, and that a different mouse study found that deleting CCR5 improved their cognitive abilities.
“Have you inadvertently caused an enhancement?” Lovell-Badge asked He after the talk. The mouse study needed verification, He replied. “I am against using genome editing for enhancement.”
Another big safety issue is off-target effects – the risk that CRISPR causes unintended, harmful mutations elsewhere in the genome. To try to prevent this, He’s team sequenced the entire genomes of both parents. They then removed 3 to 5 cells from each of the edited embryos before implantation in the mother and fully sequenced them, too, to check for unwanted mutations.
Comparing the genomes revealed several new mutations in the two edited embryos for which results have been released. Only one of these mutations – found in the embryo of the girl nicknamed “Lulu” – might be due to CRISPR, He concluded. It’s possible that this may be the case, because every individual has up to 100 new mutations by chance anyway.
The possible off-target mutation was judged by the team to be harmless because it is in a region of DNA that is far from any genes. According to the slides He presented, the parents were told about it and decided to proceed.
But CRISPR expert Gaetan Burgio of the Australian National University tweeted that the checks for off-target mutations were not good enough. For instance, they would not have detected any very large deletions of DNA, he said.
The final big safety issue with using CRISPR on embryos is something called mosaicism. If the eggs started dividing before the gene editing took place, the twin girls might have a mixture of cells with and without the edit. Whether they do or not was unclear from He’s talk.
Tests on the placenta and umbilical cord blood and tissue found exactly the same mutations in each sample for both twins, the slides reveal. But the potential off-target mutation was found only in the cells taken from the embryo and not in later samples, which does imply mosaicism. And Burgio told New Scientist that the results suggest both twins are mosaics. “I can’t believe they went ahead and implanted the embryos,” he says.
Mosaicism is an issue for two reasons. Firstly, if an embryo is a mosaic then removing a few cells for testing is not enough to check the health and status of an embryo. Secondly, if Lulu’s immune cells developed from non-edited cells, they would still be completely vulnerable to HIV.
We know the other twin, “Nana”, is definitely still completely vulnerable to HIV. She has a 15-DNA-letter long deletion in one of the two copies of the CCR5 gene that probably will not be enough to disable the protein. And the other copy was not edited at all.
Questions had been raised over why Nana’s embryo was implanted at all, but He said the parents were informed and decided to implant it.
This will definitely alter the trajectory of human life on this planet and nobody in the scientific community could predict any definitive outcome of the project.
It doesn’t need a rocket scientist to say that the same technology could be used for some nefarious objectives, not just in China but the rest of the G20 member countries, which themselves have been involved with genetic engineering far longer than the Chinese.
This is leveling up industrial medicine to greater heights.
To be fair to China, the SUSTC is disowning Dr. Jiankui HE’s work:
Southern University of Science and Technology Statement On the Genetic Editing of Human Embryos Conducted by Dr. Jiankui HE – In the Focus
On November 26, 2018, the Southern University of Science and Technology (hereafter SUSTech or the University) was informed through media news reports that Dr. Jiankui HE (who has been on no-paid leave from February 2018 through January 2021) released a public announcement that he has carried out genetic editing on human embryos.
The University was deeply shocked by this event and has taken immediate action to reach Dr. Jiankui HE for clarification. Dr. Jiankui HE’s previous affiliation, the Department of Biology (hereafter the Department) called an emergency meeting of the Department Academic Committee.
Based on the information collected by the time of this release, SUSTech hereby wishes to make the following preliminary statement:
1. The research was conducted outside of the campus and was not reported to the University nor the Department. The University and the Department were unaware of the research project and its nature.
2. The SUSTech Department of Biology Academic Committee believes that Dr. Jiankui HE’s conduct in utilizing CRISPR/Cas9 to edit human embryos has seriously violated academic ethics and codes of conduct.
3. All research conducted at SUSTech is required to abide by laws and regulations, and comply with international academic ethics and codes of conduct.
The University will call for international experts to form an independent committee to investigate this incident, and to release the results to the public. – Southern University of Science and Technology